Coding

Part:BBa_K4853002:Design

Designed by: Bhavya Guduru   Group: iGEM23_Virginia   (2023-10-12)


WELQ + V8 cleavage site + nisA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

V8 site was included by making the amino acid substitution R23E. The V8 protease originates from Staphylococcus aureus, one of the main pathogens in cellulitis infections.


Source

The L. lactis gene sequence was found through the NCBI nucleotide database[1].

[1] Lactococcus lactis strain N8 chromosome, complete genome - nucleotide - NCBI. National Center for Biotechnology Information. U.S. National Library of Medicine.

References